Table 3 Specific activities in cell extracts with various phosphomonoesters Each reaction mixture contained 10 mM substrate, 25 mM MgCl2, 0.1% BSA in 100 mM imidazole/HCl (pH 6.5)
From: The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5'-nucleotidase
Substrates | Activity (unit × 103/mg protein) | |
|---|---|---|
Knock-out strain | Wild-type strain | |
IMP | 0.4 | 16.6 |
AMP | 1.2 | 0.7 |
GMP | 0.6 | 0.5 |
UMP | 0.5 | 0.4 |
CMP | 0.4 | 0.3 |
Phenylphosphate | 11.7 | 11.2 |
1-Glycerophosphate | 106.4 | 98.8 |