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Table 3 Steady state kinetic analysis of wild type and mutant riboflavin synthases

From: Riboflavin synthase of Schizosaccharomyces pombe. Protein dynamics revealed by 19F NMR protein perturbation experiments

enzyme

vmax [nmol mg-1 min-1]

KM [μM]

wild type

158 ± 2.9

5.7 ± 0.24

S146A

183 ± 9.5

3.8 ± 0.40

S146C

179 ± 7.0

8.9 ± 1.06

C48S

27 ± 1

1.1 ± 0.13

C48A

< 0.1

 

C48M

< 0.1

 
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BMC Biochemistry

ISSN: 1471-2091

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