Table 1 Bacterial strains and plasmids

E. coli strain

XL-1-Blue

recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac[F', proAB, lacI^qZΔ M15, Tn10(tet^r)]

34

M15 [pREP4]

lac, ara, gal, mtl, recA⁺, uvr⁺, [pREP4: lacI, kan^r]

43

BL21 (DE3)

F^-, ompT, hsdSB, (r^-B, m^-B), gal, dcm (DE3)

44

Plasmids

pNCO113

expression vector

36

pT7-7

expression vector

45

pNCO-SSP-Rosy-WT

vector expressing the wild type SSP-Rosy

this study

pT7-SSP-Rosy-C48S

vector expressing a C48S mutated SSP-Rosy

this study

pT7-SSP-Rosy-C48A

vector expressing a C48A mutated SSP-Rosy

this study

pT7-SSP-Rosy-C48M

vector expressing a C48M mutated SSP-Rosy

this study

pT7-SSP-Rosy-S146A

vector expressing a S146A mutated SSP-Rosy

this study

pT7-SSP-Rosy-S146C

vector expressing a S146S mutated SSP-Rosy

this study