Figure 6
19F NMR signals of 6,7-bis(trifluoromethyl)-8-ribityllumazine hydrate (epimer A; Compound 6a). A, lumazine protein of P. leiognathi (0.14 mM protein, 0.23 mM 6a); B, recombinant N-terminal domain of E. coli riboflavin synthase (0.75 mM protein, 0.56 mM 6a); C, riboflavin synthase of E. coli (0.5 mM protein, 3.34 mM 6a); D, riboflavin synthase of B. subtilis (0.13 mM protein, 0.41 mM 6a); E, riboflavin synthase of S. pombe (0.26 mM protein, 0.60 mM 6a); x, impurities. For other details see legend to Fig. 5.