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Figure 2 | BMC Biochemistry

Figure 2

From: Probing the interface in a human co-chaperonin heptamer: residues disrupting oligomeric unfolded state identified

Figure 2

A. Polyacrylamide gel showing cpn10 variants after treatment with cross-linking agent gluteraldehyde (lanes 3–5). All reactions included 60 μM total protein (5 μg total protein loaded in each lane). Lane 1, marker (200.0 kDa; 116.0 kDa; 97.4 kDa; 66.3 kDa; 55.4 kDa; 36.5 kDa; 31.0 kDa, 21.5 kDa; 14.4 kDa; 6.0 kDa); Lane 2, wild-type cpn10 without cross-linking agent; Lane 3, wild-type cpn10; Lane 4, Val100Gly cpn10; Lane 5, Phe8Gly cpn10. B. Gel filtration traces for wild-type cpn10 (light blue), Val100Gly cpn10 (green), and Phe8Gly cpn10 (blue). The column was calibrated with molecular weight globular standards (dashed line): Ribonuclease A, 13,700 Da; Chymotrypsinogen A, 25,000 Da; Ovalbumin, 43,000 Da; Bovine Serum Albumin, 67,000 Da; Blue Dextran 2000, » 2,000,000 Da.

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BMC Biochemistry

ISSN: 1471-2091

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