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Figure 4 | BMC Biochemistry

Figure 4

From: Comparative inhibition by substrate analogues 3-methoxy- and 3-hydroxydesaminokynurenine and an improved 3 step purification of recombinant human kynureninase.

Figure 4

Inhibition of rat hepatic kynureninase by 3-hydroxydesaminokynurenine (4) Primary Lineweaver Burk (L/B) plot of kinetic data for inhibition of rat hepatic kynureninase by 3-hydroxydesaminokynurenine (4) (I = 0 (■); I = 50 nM (▲) I = 100 nM (▼); I = 200 nM (◀ ▶); I = 400 nM (●); I = 600 nM (□)) depicting mixed inhibition. s = substrate (3-hydroxykynurenine) and v = specific activity. The inset is a secondary plot of slope against [I] to determine the Ki (40 nM). The slopes were calculated from a L/B plot (n = 3).

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BMC Biochemistry

ISSN: 1471-2091

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