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Figure 2 | BMC Biochemistry

Figure 2

From: Comparative inhibition by substrate analogues 3-methoxy- and 3-hydroxydesaminokynurenine and an improved 3 step purification of recombinant human kynureninase.

Figure 2

Discontinuous gel electrophoresis of human kynureninase. 10% SDS-Page gel image of purified recombinant kynureninase (20 μg) at 52.4 kDa in the presence of PLP. This NuPAGE Novex Bis-Tris Gel (prepacked) was produced using the Xcell SureLock Mini-Cell from Invitrogen. The pertained Mark 12 standards were also acquired from Invitrogen. Run conditions were 200 V (constant)/35 minute in MES buffer with expected current of 100–125 mA/gel at start going to 60–80 ma/gel at the end.

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BMC Biochemistry

ISSN: 1471-2091

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