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Figure 6 | BMC Biochemistry

Figure 6

From: Nuclear annexin II negatively regulates growth of LNCaP cells and substitution of ser 11 and 25 to glu prevents nucleo-cytoplasmic shuttling of annexin II

Figure 6

Distribution of GFP and GFP-fused wild type or NES mutant annexin II in LNCaP cells. LNCaP cells expressing GFP, GFP-wild type annexin II (GFP-WT), and GFP-NES mutant annexin II (GFP-NES) were fractionated into cytosolic extract (CE) and nuclear extract (NE). Each extract was subjected to SDS-PAGE and immunoblot with anti-GFP (Panel A) and anti-PGK antibody (panel B). Cytosolic and nuclear extracts from the GFP-NES cells were subjected to potato acid phosphatase (PACP) treatment as described in materials and methods, and an immunoblot analysis was performed for annexin II (Panel C).

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BMC Biochemistry

ISSN: 1471-2091

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