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Figure 3 | BMC Biochemistry

Figure 3

From: Glycosaminoglycans in human retinoblastoma cells: Heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions

Figure 3

Spectrophotometric assays for heparin and HS-like molecules. Heparin and HS were degraded with heparinase and heparitinase, respectively. Proteins in CM concentrated 10-fold and CMPEDF were digested first with subtilisin. Protease-treated CM and CMPEDF were reacted with heparinase and heparitinase. The appearance of degradation products (Δ4-hexuronate) was measured by absorbance at 235 nm (Extinction coefficient = 5500). Panels A and B show heparinase activity and panels C and D show heparitinase activity. Time course and concentration curves of enzymes using their respective substrates are shown in panels A and C. Panels B and D correspond to reactions with CM and CMPEDF.

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BMC Biochemistry

ISSN: 1471-2091

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