Mitochondria from cultured cells derived from normal and thiamine-responsive megaloblastic anemia individuals efficiently import thiamine diphosphate

Table 1 Kinetic constants for the high affinity component of ThDP uptake by mitochondria isolated from various cell types.

K_m, micromolar; V_max, pmol per mg protein per min. Values for normal lymphoblasts are derived from a Lineweaver-Burk plot using the mean velocity values averaged from 4 independent experiments. Two independent experiments were performed for TRMA lymphoblasts, fibroblasts, and neuroblastomas, and one experiment was performed for glyB cells.

ISSN: 1471-2091