Table 2 Spectral characteristics for some mutants of asCP and DsRed.
From: Interconversion of Anthozoa GFP-like fluorescent and non-fluorescent proteins by mutagenesis
Wild type protein | Mutant | Absorption max, nm | Emission max, nm | Extinction coefficient, M-1cm-1 | Quantum yield |
|---|---|---|---|---|---|
asCP | wild typea | 568 | 595 | 56,000 | << 0.001 |
| Â | A148S | 572 | 597 | 15,000 | 0.012 |
| Â | S165V | 583 | 620 | 18,000 | 0.008 |
DsRed | wild typeb | 558 | 583 | 75,000 | 0.70 |
| Â | S148A | 568 | 591 | 73,000 | 0.45 |
| Â | S203A | 562 | 583 | 74,000 | 0.70 |
| Â | S148A, S203A | 574 | 593 | 73,000 | 0.29 |
| Â | S148A, K167M | 572 | 595 | 80,000 | 0.22 |
| Â | S148A, K167M, S203A | 574 | 596 | 104,000 | 0.09 |
| Â | S148A, I165S, S203A | 574 | 595 | 68,000 | 0.06 |
| Â | S148A, I165S, K167M, S203A | 552 | 593 | 77,000 | 0.007 |
| Â | S148C, I165N, S203A | 574 | 591 | 80,000 | 0.009 |
| Â | S148C, I165N, K167M, S203A | 561 | 600 | 57,000 | << 0.001 |