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Table 2 Metal Content of Wild-type L1 and L1 mutants

From: Probing substrate binding to Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia by using site-directed mutagenesis

Sample Zn(II) Content (moles Zn(II)/mole of enzyme)a
Wild-type 1.9 ± 0.2
S224A 1.8 ± 0.2
S224D 1.7 ± 0.3
S224K 1.0 ± 0.1
Y228A 1.8 ± 0.1
Y228F 1.7 ± 0.3
F158A 1.5 ± 0.2
I164A 1.6 ± 0.2
N233L 1.8 ± 0.2
N233D 1.5 ± 0.1
  1. a The final dialysis buffer was used as a blank, and the Zn(II) content in the final dialysis buffers was shown to be < 0.5 μM in separate ICP measurements.