Skip to main content

Table 2 Metal Content of Wild-type L1 and L1 mutants

From: Probing substrate binding to Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia by using site-directed mutagenesis

Sample

Zn(II) Content (moles Zn(II)/mole of enzyme)a

Wild-type

1.9 ± 0.2

S224A

1.8 ± 0.2

S224D

1.7 ± 0.3

S224K

1.0 ± 0.1

Y228A

1.8 ± 0.1

Y228F

1.7 ± 0.3

F158A

1.5 ± 0.2

I164A

1.6 ± 0.2

N233L

1.8 ± 0.2

N233D

1.5 ± 0.1

  1. a The final dialysis buffer was used as a blank, and the Zn(II) content in the final dialysis buffers was shown to be < 0.5 μM in separate ICP measurements.
Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us