Figure 3

(A) Stopped-flow fluorescence of wild-type L1 with nitrocefin. In a typical reaction, 5–10 μM wild-type L1 was mixed with various concentrations of nitrocefin, and the reaction was monitored for up to 1 second at 10°C, using the conditions described in Materials and Methods. The data were fitted to a double exponential using SigmaPlot v. 6.10.(B) Plot of observed rate constant versus concentration of nitrocefin. Solid lines were fitted to the data as described in Materials and Methods.