Figure 6

Inhibition of PKG partially prevents the DETA/NO-induced down-regulation of β₁ subunit protein levels. Cells were incubated with either: vehicle (control), 50 μmol/L DETA/NO, 10 μmol/L ODQ, 10 μmol/L ODQ plus 50 μmol/L DETA/NO, 15 μmol/L Rp-8-BrPET-cGMPS (Rp-PET), 15 μmol/L Rp-8-BrPET-cGMPS (Rp-PET) plus 50 μmol/L DETA/NO, or 10 μmol/L Sp-8-BrPET-cGMPS (Sp-PET) plus 50 μmol/L DETA/NO for 48 hours. ODQ, Rp-PET and Sp-PET were added 30 min before DETA/NO. Proteins (30 μg) from each group were subjected to electrophoresis and blotting. sGC was immunodetected and the band corresponding to the β₁ subunit quantified. Results are expressed as the percentage (%) of the amount of β₁ subunit in cells subjected to different treatment with respect to the amount in untreated cells. The figure shows the results given as mean ± SE of five experiments performed with different cultures. Significant differences compared with non-treated cells (control) is indicated by ^***p < 0.001 (Student's t test). ⁺⁺p < 0.01 represents significant difference between DETA/NO-treated cells and DETA/NO plus Rp-PET-treated cells.