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Figure 1 | BMC Biochemistry

Figure 1

From: Prolonged exposure of chromaffin cells to nitric oxide down-regulates the activity of soluble guanylyl cyclase and corresponding mRNA and protein levels

Figure 1

Concentration-dependence curves for DEA/NO-stimulated cGMP accumulation on chromaffin cells. Cells were incubated for 30 min in Locke's solution containing 0.5 mM IBMX. Then they were stimulated for 10 min with the indicated DEA/NO concentrations and cGMP measured by radioimmunoassay. Results expressed as pmol /106 cells, corresponding to the mean ± standard error of three experiments performed in triplicate, with different cultures. DEA/NO was freshly prepared every day in NaOH 0.01 N and its concentration checked by measuring the absorbance at 250 nm. Data were fit to sigmoidal representation by using Microcal Origing program.

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BMC Biochemistry

ISSN: 1471-2091

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