Skip to main content
Figure 4 | BMC Biochemistry

Figure 4

From: Delineation of RAID1, the RACK1 interaction domain located within the unique N-terminal region of the cAMP-specific phosphodiesterase, PDE4D5

Figure 4

Pull down assays to probe the interaction between PDE4D5 and RACK1. Pull down assays between RACK1 and PDE4D5 mutants were done as described in Methods. Shown are immunoblots for VSV-epitope tagged forms of full length PDE4D5 detected using an anti-VSV monoclonal antibody. Tracks 1 to 5 are immunoblots of cell lysates showing that equal amounts of immunoreactive material were taken for these pull-down studies. Tracks 6 to 10 are blots showing the 'pull-downs', namely material that is found associated with glutathione agarose-immobilized GST-RACK1. Full length PDE4D5 forms were used as either wild type (tracks 1, 6) or the mutant forms Leu33Asp (tracks 2, 7), Arg34Ala (tracks 3, 8), Leu37Asp:Leu38Asp (tracks 4, 9) and Leu37Glu:Leu38Glu (tracks 5, 10). These data are typical of experiments done at least three times.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us