Figure 5

ATP hydrolysis is required for the DNA binding activity of p35. Gel shift assays were performed using ³²P labelled ARS fragment either in the presence of ATP (lanes 3–7) or ATPγS (lanes 8–12) or both (lanes 13–14). A strong band shift was observed in the presence of ATP whereas ATPγS completely inhibited the binding. The inhibition can be reversed by adding back ATP in the reaction mixture.