Table 3 Kinetic parameters of hAps1, hAps1-39N and hAps2
Enzyme | Substrate | Km (μM) | kcat (s-1) | kcat/Km (103M-1s-1) |
|---|---|---|---|---|
hAps1 | Ap6A | 13 ± 5 | 0.17 ± 0.04 | 13.1 |
Ap5A | 37 ± 9 | 0.4 ± 0.1 | 10.8 | |
PP-InsP5 (n = 2) | 4 | 0.20 | 50 | |
hAps1-39N | Ap6A | 11 ± 3 | 0.16 ± 0.02 | 14.5 |
Ap5A | 50 ± 10 | 0.4 ± 0.1 | 8.0 | |
PP-InsP5 (n = 2) | 0.3 | 0.09 | 290 | |
hAps2 | Ap6A | 19 ± 1 | 0.20 ± 0.01 | 10.5 |
Ap5A | 50 ± 10 | 0.8 ± 0.2 | 16.0 | |
PP-InsP5 | 1.3 ± 0.6 | 0.14 ± 0.02 | 110 |