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Figure 3 | BMC Biochemistry

Figure 3

From: Insertion of a small peptide of six amino acids into the β7–β8 loop of the p51 subunit of HIV-1 reverse transcriptase perturbs the heterodimer and affects its activities

Figure 3

DNA binding affinity of mutant HIV-1 RT carrying insertion in the β7–β8 loop of either or both the subunits. The 49-mer DNA primed with 5'-32P labeled 21-mer DNA primer was incubated with varying concentrations of the individual enzyme at 4°C for 10 min. The mixture was electrophoresed under non-denaturing conditions on a 6% (w/v) polyacrylamide gel and analyzed on phosphorImager (Left panel). The positions of the free template primer (TP) and enzyme bound template-primer (E-TP binary complex) are indicated for the wild type enzyme. The percent of E-TP complex formed as a function of enzyme concentration was plotted for determining the Kd values (Right panel).

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BMC Biochemistry

ISSN: 1471-2091

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