Figure 2
From: Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins
Purified proteins used in this study. Purified proteins were run on SDS polyacrylamide gels, and visualized with either Coomassie Blue (a-c, e-h) or silver staining (d). a, Nucleoplasmin purified from Xenopus eggs. b, ORC purified from Xenopus eggs. c, Recombinant Xenopus Cdc6 purified from baculovirus-infected insect cells. d, RLF-B/Cdt1 purified from Xenopus eggs. e, Recombinant Xenopus ΔCdt1 purified from E. coli. f, Mcm(2+4+6+7) purified from Xenopus eggs. g, Mcm(3+5) purified from Xenopus eggs. h, Recombinant Xenopus geminin purified from E. coll.