Figure 10

Sensitivity of BN cells to GCDCA treatment. The cytotoxic potential of GCDCA in BN and McNtcp.24 cells was assessed by measuring LDH release and caspase 8 activity. (A) LDH activity in the medium of cells treated with 100 μM GCDCA for 4 h. The values are relative to the LDH activity in the medium of GCDCA-treated McNtcp.24 cells (100 %). (B, C) Cells were treated with 50 μM GCDCA for 1 h and with 10 μM for 2 and 4 h. Caspase 8 activity was then assayed in cellular extracts. The values represent means ± SD (n = 3). The experiment in (A) was repeated three times and the experiments in (B) and (C) were repeated twice with similar results.