Figure 4
Analysis of helicase inhibition kinetics. Concentration curves of compounds A. netropsin, B. nogalamycin, C. daunorubicin, and D. ethidium bromide. In each panel, the autoradiogram of the assay is shown on the left and the quantitative data on the right side. Lane C in all the panels is control without enzyme and lane B in all the panels is heated substrate. Lanes 1-7 are reactions with enzyme in the presence of 0, 0.5, 1, 2, 3, 4 and 5 μM of compounds labeled below the autoradiogram.