Table 1 Primers designed and used in this study
Construct | Mutation | Template | Primer name | Primer sequence |
|---|---|---|---|---|
HY11W51A | W51A | pHmuY11 | HY11W51AF | 5’- cgatgcttcgaaatacgaaacgGCgcagtatttctctttttccaaagg-3’ |
HY11W51AR | 5’- cctttggaaaaagagaaatactgcGCcgtttcgtatttcgaagcatcg-3’ | |||
HY11W73A | W73A | pHmuY11 | HY11W73AF | 5’- ctataagaacgatttgaacGCGgacatggctcttca-3’ |
HY11W73AR | 5’- gcggtgaagagccatgtcCGCgttcaaatcgttcttatag-3’ | |||
HY11W161A | W161A | pHmuY11 | HY11W161AF | 5’- agggatttgcttcaggtggtGCGctggaattctctcacggtc-3’ |
HY11W161AR | 5’- gaccgtgagagaattccagCGCaccacctgaagcaaatccct-3’ | |||
HY11W51Y | W51Y | pHmuY11 | HY11W51YF | 5’- cgatgcttcgaaatacgaaacgTATcagtatttctctttttccaaagg-3’ |
HY11W51YR | 5’- cctttggaaaaagagaaatactgATAcgtttcgtatttcgaagcatcg-3’ | |||
HY11W73Y | W73Y | pHmuY11 | HY11W73YF | 5’- ctataagaacgatttgaacTATgacatggctcttcaccgc-3’ |
HY11W73YR | 5’- gcggtgaagagccatgtcATAgttcaaatcgttcttatag-3’ | |||
HY11W161Y | W161Y | pHmuY11 | HY11W161YF | 5’- agggatttgcttcaggtggtTATctggaattctctc-3’ |
HY11W161YR | 5’- gaccgtgagagaattccagATAaccacctgaagcaaatccct-3’ |