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Figure 5 | BMC Biochemistry

Figure 5

From: The basic amino acids in the coiled-coil domain of CIN85 regulate its interaction with c-Cbl and phosphatidic acid during epidermal growth factor receptor (EGFR) endocytosis

Figure 5

Interactions of CIN85 with Vps proteins. (A) Interactions of CIN85 and CIN85-ΔCC with CHMP4B. Flag-tagged CIN85 and eGFP-tagged CHMP4B were co-expressed in HEK293 cells and Flag-CIN85 was immunoprecipitated by anti-Flag antibody. CHMP4B in immunoprecipitated sample was detected by Western blot. (B) Interactions of CIN85 and CIN85-ΔCC with Vps4 and Vps4m (ATPase negative mutant). Flag-tagged CIN85 and eGFP-tagged Vps4 were co-expressed in HEK293 cells and Flag-CIN85 was immunoprecipitated by anti-Flag antibody. Vps4 in immunoprecipitated sample was detected by Western blot. (C) Interactions of CIN85 and CIN85-ΔCC with different Vps proteins. The tagged CIN85 and Vps protein were co-expressed in HEK293 cells and CIN85 was immunoprecipitated. Vps proteins were detected by Western blot. (D) Co-localization of CIN85 and CIN85-ΔCC with CHMP4B. Flag-tagged CIN85 was co-expressed with eGFP-tagged CHMP4B in HEK293 cells. Cells were fixed with 4% PFA and observed by confocal microscopy. Τhe bar is 10 μm. (E, F) Co-localization of Flag-tagged CIN85 and CIN85-ΔCC with eGFP-tagged Vps4 and Vps4m.

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BMC Biochemistry

ISSN: 1471-2091

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