Figure 3From: A broad survey reveals substitution tolerance of residues ligating FeS clusters in [NiFe] hydrogenasePurified protein activity assay . Left: Hydrogen evolution activities of G1, C258D, and C295D after purification using IMAC/strep-tactin tandem purification. Error bars represent the standard error of the mean activity. Right: anti-HynL and anti-strep western blots of enzyme samples.Back to article page