Figure 6
From: Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7
Identification of stable Mcm complexes and helicase activity of Mcm4/6/7 hexamers. (A) Gel filtration chromatography profiles of purified complexes of Mcm4, 6 and 7. Gel filtration analysis was carried out a described under “Materials and Methods”. Asterisk: Gel filtration profile of Mcm4/6/7 hexamers expressed and purified from insect cells in our laboratory. (B) SDS-PAGE analysis of purified complexes of Mcm4, 6 and 7 from the gel filtration fractions shown in Panel C. (C) Helicase assay results of the Mcm4/6/7 hexamers. No protein added in lane1 and 2. B, boiled substrate; UB, un-boiled substrate; lane 3 and 5, 100 ng protein added; lane 4 and 6, 200 ng protein added; Asterisk, Mcm4/6/7 hexamers expressed and purified from insect cells.