Figure 4From: The mononuclear metal center of type-I dihydroorotase from aquifex aeolicusEffect of excess zinc on the DHO-ATC steady state kinetics. A dihydroorotate saturation curve of DHO-ATC (Methods; 250 mM Tris acetate, pH 8.3, 72°C) was carried out in the presence of a 5-fold molar excess of ZnCl2 (○) or in the absence of exogenous zinc (●). The kinetic parameters are summarized in Table 4.Back to article page