Table 1 Purification of P. americana protease
From: Characterization of a chemostable serine alkaline protease from Periplaneta americana
Purification | Volume (mL) | Activity (μM/mL/min) | Total activity (μM) | Specific activity (μM/mg/min) | Total protein (mg) | Purification fold | Purification yield % |
|---|---|---|---|---|---|---|---|
Step | |||||||
Crude | 50 | 4.117 | 164.68 | 1.52 | 250 | 1 | 100 |
Acetone ppt | 6 | 1.116 | 6.669 | 2.2 | 30 | 1.4 | 4 |
Gel filtration | 5 | 6.5 | 32.5 | 6.5 | 25 | 4.2 | 19 |