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Table 1 Purification of P. americana protease

From: Characterization of a chemostable serine alkaline protease from Periplaneta americana

Purification Volume (mL) Activity (μM/mL/min) Total activity (μM) Specific activity (μM/mg/min) Total protein (mg) Purification fold Purification yield %
Step
Crude 50 4.117 164.68 1.52 250 1 100
Acetone ppt 6 1.116 6.669 2.2 30 1.4 4
Gel filtration 5 6.5 32.5 6.5 25 4.2 19
  1. Purification fold, percentage yield and activity of each purification step.