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Figure 8 | BMC Biochemistry

Figure 8

From: Detrimental effect of the 6 His C-terminal tag on YedY enzymatic activity and influence of the TAT signal sequence on YedY synthesis

Figure 8

X-band EPR spectra of as-isolated YedY. (A) C-ter tagged YedY, (B) N-ter tagged YedY and (C) YedY after cleavage of the tag. Conditions: T = 55 K, microwave frequency = 9,413 GHz, microwave power = 1 mW, modulation amplitude = 0.5mT. The spectral simulation (dashed lines) yields (A) 100% of species 1, (B) 67% / 33% of species 1 and 2 respectively, (C) 100% of species 2.

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BMC Biochemistry

ISSN: 1471-2091

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