Table 5 Relative cleavage frequencies of wild type and mutated CsnN174 chitosanases from (GlcN)6 hydrolysis at 50% of substrate depletion calculated from data on Figure 7
Symmetrical cleavage | Asymmetrical cleavage | |||
|---|---|---|---|---|
Enzyme | 6 → 3 + 3 | 4 → 2 + 2 | Total | 6 →4 + 2 |
WT | 5,14 (60%) | 0,28 (3%) | 5,42 (63%) | 3,19 (37%) |
R42E | 5,66 (66%) | 0,65 (8%) | 6,21 (74%) | 2,23 (26%) |
R42K | 5,73 (65%) | 0,53 (6%) | 6,26 (71%) | 2,60 (29%) |