Table 4 Specific activities of wild type and mutated CsnN174 chitosanases towards chitosans with different degrees of N -deacetylation
Specific activity of chitosanase (U/mg) | |||
|---|---|---|---|
Chitosan degree ofN-deacetylation (%) | WT | R42K | R42E |
97 | 73± 2 | 1.37 ± 0.03 | 0.816 ± 0.006 |
94 | 69 ± 1 | 1.42 ± 0.04 | 0.854 ± 0.008 |
86 | 56 ± 2 | 1.48 ± 0.05 | 0.91 ± 0.01 |
84 | 52.9 | 1.4 | 0.9 |
72 | 13.0 ± 0.6 | 1.19 ± 0.04 | 0.69 ± 0.01 |
65 | 7.5 ± 0.9 | 0.73 ± 0.01 | 0.464 ± 0.005 |
62 | 8.1 ± 0.5 | 0.63 ± 0.04 | 0.35 ± 0.01 |