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Table 3 Transition temperatures of thermal unfolding of wild type and R42-mutated CsnN174 chitosanases in the absence or presence of two chitosans with different degrees of N -deacetylation

From: A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding

  

T m (°C)

Enzyme

Enzyme only

Enzyme + chitosan (DDA 84%)

Enzyme + chitosan (DDA 99%)

Chitosan (DDA 84%)

Chitosan (DDA 99%)

WT

48.8

53.4

53.2

4.6

4.4

R42E

43.2

59.5

62.5

16.3

19.3

R42K

47.7

62.5

66.1

14.8

18.4