From: A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding
T m (°C)
Enzyme
Enzyme only
Enzyme + chitosan (DDA 84%)
Enzyme + chitosan (DDA 99%)
Chitosan (DDA 84%)
Chitosan (DDA 99%)
WT
48.8
53.4
53.2
4.6
4.4
R42E
43.2
59.5
62.5
16.3
19.3
R42K
47.7
66.1
14.8
18.4