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Table 2 Kinetic parameters of purified wild-type and mutant CsnN174

From: A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding

Enzyme

Km(μg/ml)

kcat(min-1)

Ki(μg/ml)

kcat/ Km(min*ml/μg)

Wild-type

26.1

642.1

1547

24.6

R42E

441.7

96.7

263

0.22

R42K

204.1

55.5

612

0.27

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BMC Biochemistry

ISSN: 1471-2091

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