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Table 1 Specific activities of purified wild-type and mutant CsnN174

From: A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding

Enzyme

Specific activity (units per mg protein)

Wild type*

52.9

D40G*

1.6

R42E

0.9

R42K

1.4

D40G+R42E

0.0012

D40G+R42K

0.018

  1. * Data from ref.[17].