Figure 5From: A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate bindingThermal unfolding curves of wild type or mutant chitosanases in the absence or presence of chitosan. (A) wild type. (B) R42K chitosanase. (C) R42E chitosanase. (a) no chitosan. (b) 82.5% DDA chitosan. (c) 99% DDA chitosan. The unfolding process was monitored by intrinsic tryptophan fluorescence.Back to article page