Figure 4

Human macrophages were transfected with either the negative control or PKC-θ-siRNA and then incubated without or with TNFa at 10 ng/ml, 10 ng/ml for 96 h. The Relative protein fold change of (A) ABCA1, (B) ABCG1, (C) LXRa (D) CD-36, (E) SR-A were shown in Figure 4. (G) ABCA1, ABCG1, LXRa and (H) CD-36, SR-A protein expression were measured using Western immunoblotting techniques. Human macrophages were transfected with either the negative control or PKC-θ-siRNA and then incubated for 96 h. (F) Relative protein fold change of PKC-θ in human macrophages transfected with either the negative control or PKC-θ-siRNA. (I) PKC-θ protein expression were measured using Western immunoblotting techniques. Similar results were obtained in three independent experiments. Data are presented as mean ± SEM, *P < 0.05 vs control.