Table 2 Oligonucleotides used in this study
From: Construction of a chimeric thermoacidophilic beta-endoglucanase
Name | Sequence (5′ – 3′) |
|---|---|
celA_BsaI.for | AGGTCTCGCATGGTGGTACTGATGACAAAACCGGGAA |
celA_HindIII.rev | AAAGCTTTCATTCTCTCACCTCCAGATCAATAGAG |
1_1949_celA.rev | CAGGGTTTTCTGCCCACATAAAACTCGGGATAAC |
2_CelA_1949.for | GTTATCCCGAGTTTTATGTGGGCAGAAAACCCTG |
3_CelA_1949.rev | CTTCCAAACTCG GTTCCGAACTCCCAATCCGTTA |
4_1949_CelA.for | TAACGGATTGGGAGTTCGGAACCGAGTTTGGAAG |
5_CelA_1949.rev | TTGAAATAGAACCAGACCATTATCTCAATGTCCC |
6_1949_CelA.for | GGGACATTGAGATAATGGTCTGGTTCTATTTCAA |
7_1949_CelA.rev | TAATAGTTTGTAATGTTAGCAGAACTCGAAAGAG |
8_CelA_1949.for | CTCTTTCGAGTTCTGCTAACATTACAAACTATTA |
T7-Promotor | TAATACGACTCACTATAGGG |
T7-Terminator | GCTAGTTATTGCTCAGCG |