Table 1 pH and temperature optimum as well as kinetic parameters of SSO1949[9], CelA and the hybrid protein (this work)
From: Construction of a chimeric thermoacidophilic beta-endoglucanase
Optimal pH | Optimal temperature | kcat[s-1] | KM[μM] | kcat/KM[s-1μM-1] | |
|---|---|---|---|---|---|
80°C1 μM cellohexaoside | 1 μM cellohexaoside | 80°C | 80°C | 80°C | |
SSO1949 | 1.8 | 80°C | 0.5 | 2.0 | 0.25 |
CelA | 4.5 | 95°C | 19 ± 0.9 | 5.5 ± 0.8 | 3.5 |
CelA-SSO1949-CelA | 3 | 85°C | 0.39 ± 0.01 | 1.74 ± 0.15 | 0.23 |