Figure 5

Michaelis-Menten kinetics of the chimeric enzyme CelA-SSO1949-CelA at pH 3 and 80°C. The initial rates are shown as a function of fluorescent cellohexaoside concentration. The points are measured rates and the line is the least-squared minimized fit of the data to the Michealis-Menten equation with k_cat = 0.39 ± 0.01 s^-1 and K_M = 1.74 ± 0.16 μM.