Figure 4
From: Construction of a chimeric thermoacidophilic beta-endoglucanase
Purification of recombinant hybrid protein CelA-SSO1949-CelA. (A) Protein fractions were analysed by SDS-PAGE followed by Coomassie Blue staining: whole cell extract (lane WCE), crude extract (lane CE), Triton X extract (lane T), Urea extract at 5, 6 and 8 M urea, respectively (lanes 5MU, 6MU, 8MU). (B) Pooled and dialyzed protein fraction after refolding and hydrophobic interaction chromatography.