Figure 3

Model of chimeric enzyme CelA|SSO1949|CelA. A model structure of the hybrid CelA-SSO1949-CelA (A) and of the hybrid SSO1949-CelA-SSO1949 (B) using the structure of CelA (3AMH) as template. The sequence alignment was performed with HHpred [15] and the structure calculation was performed with Modeller [16]. The part derived from the parent enzyme CelA is in purple; whereas the SSO1949 part is shown in cyan. The catalytic glutamate residues are shown in orange sticks. It can be clearly seen that the chimeric enzymes consists of two distinct halves derived from both parent proteins. The structures are visualised with Chimera [17].