Figure 2

Calculation of disruption energies for chimeric enzymes. The disruption energies were calculated in dependence of the two recombination sites for a chimeric protein with the structure protein A - protein B - protein A. The first recombination site is on the abscissa the second on the ordinate; the calculated disruption energies are visualized as a heat map. A: energies for the chimera CelA-SSO1949-CelA. B: energies for the chimera SSO1949-CelA-SSO1949. The triangles and boxes along the axes represent beta-sheets and alpha-helices, respectively. Red circles refer to the recombination sites selected. Abcissa and ordinate refer to alignment position. The conserved domain of GH12 starts at about alignment position 100.