Figure 1

Alignment of the cellulases SSO1949 from S. solfataricus and CelA from T. maritima. A: Alignment of SSO1949 (black letters on white background) and CelA (white on black). The signal peptide of SSO1949 is in italics and the Ser/Thr-rich region is underlined. The conserved catalytic glutamic residues and valine 63 are marked with asterisks. The four amino acids mutated at the valine 63 position are double-underlined. B: Sequence of the both fusion proteins. Black letters are amino acids derived from the parental sequence of SSO1949; white letters show residues derived from CelA.