Subcellular localization and RNA expression during development. (A) Subcellular localization studies in constitutively expressing [act15/png-eyfp]/Ax2 cells. (A) DIC image; (B) DdPNGase-EYFP image; (C) DAPI stained image; (D) merged image. The fusion protein (green) is localized maximally in the nucleus (blue) and to a lesser extent in the cytosol. (B) Temporal expression pattern of Dd PNGase during development and differentiation. Graph representing the ratio of Ddpngase to rnlA expression levels at different time points of development. High transcript level was seen at t6 hours corresponding to loose aggregate stage. (C) Transcript levels and the graph showing the normalized expression levels of Ddpngase in isolated prestalk and prespore cells. [V-vegetative; LA-loose aggregate; M-mound; S-migrating slug; EC-early culminant; C-culminant; pst-prestalk; psp-prespore].