Figure 3

Transient transfection of the ACTN1 minigene and the IR minigene. (A) Schematic representation of the ACTN1 minigene. Exons are indicated as black boxes with alternatively spliced exons indicated as gray boxes. Introns are indicated with a central narrow line. The arrows show the primer sites. (B) Results of the transient transfection experiment. COS7 cells were transiently transfected with the ACTN1 minigene with/without the CUGBP, CUGBP2 R3δ isoform, or Etr-1 expression vectors. Alternatively spliced products were analyzed by RT-PCR. (C) Densitometric analysis of the transfection products. Quantification of the alternatively spliced products was performed by densitometry. The percentage of SM exon inclusion with respect to total product is shown in graphical representation. The error bars indicate the standard error. (D) Schematic representation of the IR minigene. Exons are indicated as black boxes and alternatively spliced exons are indicated as gray boxes. Introns are shown with a central narrow line. The arrows show the primer sites. (E) Results of the transient transfection experiment. HeLa cells were transiently transfected with the IR minigene with/without the CUGBP, R3δ isoform, or Etr-1 expression vectors. Alternatively spliced products were analyzed by RT-PCR. (F) Densitometric analysis of the transfection products. Quantification of the alternatively spliced products was performed by densitometry. The percentage of exon 11 inclusion with respect to total product is shown in graphical representation. The error bars indicate the standard error.