Figure 5

Acyl-CoA specificities of avian enzymes. Relative WS (a and c) and DGAT (b) activities of isolated yeast membranes from yeast cells expressing an avian WS sequence. Assays were conducted with 10:0-OH and the given straight-chain (a and b) or branched-chain (c) acyl-CoA thioesters under standard assay conditions (a and b) or increased protein amount (10 μg) and incubation time (2 h) (c). The relative activities of each protein are given, 100% corresponds to the activities with 10:0-OH and 16:0-CoA under identical conditions. Values are mean values from at least two independent assay series. (GgWS1: 116 pmol*min^-1*mg^-1; GgWS2: 105 pmol*min^-1*mg^-1; GgWS4: 149 pmol*min^-1*mg^-1; AdWS4: 126 pmol*min^-1*mg^-1; TaWS4: 149 pmol*min^-1*mg^-1).