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Table 1 Kinetic Parameters of CaMKK Isoforms

From: In vitro substrate phosphorylation by Ca2+/calmodulin-dependent protein kinase kinase using guanosine-5′-triphosphate as a phosphate donor

CaMKK

Phosphate

K m

V max

V max / K m

isoforms

Donor

(μM)

(nmol/min/mg)

 

CaMKKα

ATP

15

625

41.7

 

GTP

444

312

0.7

CaMKKβ

ATP

15

286

19.1

 

GTP

507

77

0.2

  1. CaMKK activities were measured using GST-CaMKIα (1-293, K49E) as a substrate at 30°C in the presence of one of several concentrations (50 – 400 μM) of either ATP or GTP as described in “Methods”.
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BMC Biochemistry

ISSN: 1471-2091

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