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Table 1 Kinetic Parameters of CaMKK Isoforms

From: In vitro substrate phosphorylation by Ca2+/calmodulin-dependent protein kinase kinase using guanosine-5-triphosphate as a phosphate donor

CaMKK Phosphate K m V max V max / K m
isoforms Donor (μM) (nmol/min/mg)  
CaMKKα ATP 15 625 41.7
  GTP 444 312 0.7
CaMKKβ ATP 15 286 19.1
  GTP 507 77 0.2
  1. CaMKK activities were measured using GST-CaMKIα (1-293, K49E) as a substrate at 30°C in the presence of one of several concentrations (50 – 400 μM) of either ATP or GTP as described in “Methods”.