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Figure 4 | BMC Biochemistry

Figure 4

From: Characterization of methionine oxidation and methionine sulfoxide reduction using methionine-rich cysteine-free proteins

Figure 4

Msr activity assays by following MRP electrophoretic mobility shift on SDS-PAGE gels. 4 μg of as-isolated (lane 1) or oxidized (lanes 2 to 12) MRP1 were incubated with Msrs and DTT or Trx as described in Materials and Methods, electrophoresed on 10% SDS-PAGE gels and stained with Coomassie Blue. Migration of reduced (R) and oxidized (O) MRP1 is shown by arrows. (A) MsrA activity assay. (B) MsrB activity assay (lanes 2 to 11) and MsrA plus MsrB activity assay (lane 12). (C) MsrBA activity assay. When used separately, MsrA and MsrB partially reduced oxidized Met residues, whereas treatment with MsrBA completely restored mobility of the reduced protein.

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BMC Biochemistry

ISSN: 1471-2091

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