Figure 3
From: The role of the C8 proton of ATP in the catalysis of shikimate kinase and adenylate kinase
Shikimate kinase specific activity of WT, K15I and T17I mutant enzymes. The effect of the concentration of ATP and C8D-ATP on the specific activity and KIE of M. tuberculosis shikimate kinase.○ = WT SK using ATP, □ = WT SK using C8D-ATP, ● = T17I mutant using ATP, ■ = T17I mutant using C8D-ATP, Δ = K15I mutant using ATP, ▲ = K15I mutant using C8D-ATP. Final enzyme concentrations were: WT SK: 10 nM, T17I: 25 nM and K15I: 100 nM. The assays were run for 20 min (WT SK) or 80 min (T17I and K15I).