Figure 1

A schematic cartoon of the domain organization of DG showing the insertion of the myc-tag in different sites within the α-DG subunit. The myc-tag was inserted at the end of the signal peptide (A29), before the first residue of the solved 3D structure (PDB:1U2C) of the N-terminal region of α-DG (A60), in the middle of the flexible loop connecting the two sub-domains (Ig-like and S6-like) featured within the N-terminal region of α-DG (A170) and right before a second Ig-like domain identified within the α-DG C-terminus (K500). The C-terminal region of β-DG is fused to GFP in the myc-tagged constructs. DG numeral system is based on the majority of mammals sequences available, including the human one, all starting from an additional Met, followed by Arg (1-MRMSV-5), with respect to the murine one (3-MSV-5).